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Fig. 2-S2
Zebrafish pcnt mRNA is localized to centrosomes of mitotic retinal neuroepithelial cells in vivo.
Retinal neuroepithelial cells of 1-day-old zebrafish were subjected to pcnt FISH, anti-γ-tubulin, and anti-phospho-Histone H3 (PH3) immunostaining. Note that pcnt mRNA was localized to the centrosome of the mitotic (Cell 1, PH3+), but not of the non-mitotic cell (Cell 2). Retinas from >40 embryos were examined. Scale bars: 10 µm and 2 µm (inset).
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