Fig. 5
CaMK?II and HDACs act in the same molecular pathway leading to anterior kidney development. Embryos injected with the camk2g1 MO (1?ng), hdac 4 MO (2?ng), hdac5 MO (2?ng), camk2g1 and hdac MOs together, or the camk2g1 MO followed by bud?stage trichostatin A treatment were imaged at 72 hpf from lateral perspectives using DIC optics (A?E) or dorsal perspectives using immunofluorescence of ?1 Na+/K+ATPase (F?O). Scale bars?=?100??m. Arrowheads in I and N indicate adventitious lumens, and arrows indicate branched ducts. Convolution at 72 hpf (P) and pronephric migration (Q) as assessed by posterior ear?anterior kidney distance in ?m at 72 hpf. n?=?30?41 embryos.