Fig. 2-S3
Centra are well-formed in deltaD, deltaC, her1, her7 and hes6 segmentation clock single gene mutants.
(A, D, G, J and M) xirp2a myotome marker in situ hybridization at 27 hpf. (B, E, H, K and N) entpd5:kaede expression between 15 dpf and 20 dpf. (C, F, I, L, O) Alizarin Red bone stain in adults between 6 months and 1 year. Centra segmentation is normal in aei/deltaD, beamter/deltaC, her1, her7, and hes6 mutants. In aei/deltaD (A), bea/deltaC (D) and her7 (J) mutants, where the myotome boundaries are disordered only in the posterior trunk and tail, the neural and hemal arches of the caudal vertebrae are fused (B, C, E, F and L). All animals in lateral view with anterior to the left. Scale bars are 100 µm in A, D, G, J, M; 300 µm in B, E, H, K, N; and 0.6 mm in C, F, I, L, O).