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Fig. S6
Expression of hey family genes and notch3 in the larval and adult pallium. A,B. Expression of hey1 revealed by in situ hybridization (ISH) on a whole-mount brain (A) and pallial cross-section (B) in a wildtype 7dpf larva. C. Expression of hey1 revealed by ISH in a pallial cross-section of a 7dpf notch3-/- larva. Note the strong decrease compared to the wildtype sibling (B). D,E. Expression of notch3 revealed by in situ hybridization (ISH) on a whole-mount brain (D) and pallial cross-section (E) in a wildtype 7dpf larva. F. Pallial cross-section at equivalent levels double immunostained for BLBP (green RG cells) and PCNA (magenta, proliferating cells). Note overall that expression of hey1 and notch3 are prominent at the VZ. G. Expression of hey2 revealed by ISH in an adult pallial cross-section. No expression was detected (compared with hey1 on panel H, identical to Fig.5B). H,I. Expression of hey1 revealed by ISH in adult pallial cross-sections from a wildtype (H) and LY-treated (I) animals. Note that hey1 expression is not noticeably affected by a 48-hour LY treatment Scale bars: A-F: 20μm, G-I: 50μm.