ZFIN Image: Juan et al., 2018, Fig. S2

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Figure Caption

Fig. S2

Zebrafish myo1D controls Left-Right asymmetry in the brain, heart and viscera a-f, Asymmetric expression of lefty1 and pitx2 is impaired in the brain of MZ myo1D mutant animals. Dorsal views of lefty1 (22-somite stage) and pitx2 (25-somite stage) expression in WT (a,d) and MZ myo1D mutant (b,e) embryos. Quantification of lefty1 (c) and pitx2 (f) expression shows that asymmetric gene expression often fails to be established in mutants. g-k, MZ myo1D mutants display defects in the asymmetric expression of the nodal-related gene southpaw (spaw) in the left lateral plate mesoderm (h,k). These defects can be partially rescued by injecting wild-type myo1D RNA (i,k), but not by myo1D^tj16b mutant RNA (j,k). For the purpose of comparison, we also show the level of spaw asymmetry in wild-type embryos, using the dataset also presented in Fig. 2a-c. Scale bars: 30 μm in a,b,d,e. 50 μm in g-j.

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Acknowledgments

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