|
Fig. 3
RNAseq identification of RG quiescence and stemness markers. (A) High-magnification view of a pallial VZ area in her4:drfp;mcm5:egfp double transgenic adult, highlighting the FACS-sorted progenitor types. Cross-section processed by immunocytochemistry for RFP (magenta) and GFP (green) (left panel, magenta channel; middle panel, green channel; right panel, merge). Coloured arrows indicate the different cell types (blue, qRGs; red, aRGs; green, aNPs) (as in E). Scale bar: 10 μm. (B-D) Representative FACS dot plots showing the gating strategy. FSC/SSC plot and selected cells (B,C), which are then gated on RFP and GFP intensities (D). (E) PCA analysis on the 500 showing the greatest variability across the different FACS-sorted biological replicates (blue, qRGs; red, aRGs; green, aNPs). (F) Venn diagram illustrating the position of recovered DEGs between the different cell state comparisons and their biological interpretation.