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Fig. 3
The hars mutants display disordered connections. (A–D) In situ hybridization shows that cdh5 expression is upregulated in the brain of hars−/− at 48 and 72 hpf (arrowheads). (E,F) Antibody staining shows the Cdh5 distribution overlapping with the abnormal branching connections of CtAs, and additionally, Cdh5 appears as punctate clusters at some sprouting sites (arrowheads) in hars−/− at 48 hpf. (G,H) Pdxl2 is seen to accumulate at sprouting and connection disordered sites (arrowheads) in hars−/− at 48 hpf. (I–L) Injection of 2.5 ng cdh5 MO reduces the abnormal brain vascular connections in hars−/− at 48 hpf, but the vasculature becomes immature. Scale bars, 20 μm (A,B,G,H), 50 μm (I–L).
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