Fig. 1
ism1 is highly expressed in hematopoietic-supportive stromal cell lines.
(A) Each stromal cell line is derived from sites of hematopoietic emergence, expansion, and maintenance in adult and embryonic zebrafish; adult zebrafish kidney stroma (ZKS, derived from the adult kidney; pink[50]), zebrafish embryonic stromal trunk (ZEST, derived from the embryonic trunk; blue[48]), and caudal hematopoietic embryonic stromal tissue (CHEST, derived from the CHT; green[49]). (B) Each stromal cell line’s transcriptome was analyzed with RNA-seq and compared to a non-hematopoietic supportive stromal cell line (ZF4); transcripts were plotted as a Venn diagram demonstrating 447 conserved transcripts. (C) ism1 transcript levels for each cell line presented as reads per kilobase of transcript per million reads mapped (RPKM). See Table 1 for genes identified in this screen.