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Fig. 7
Functional Interactions between Dchs1b, Ttc28, and Aurora B in Embryonic Cleavages
(A) Co-IP assays of Flag-Dchs1b-ICD with EGFP-Aurkb and HA-Ttc28. IB, immunoblotting; IP, immunoprecipitation.
(B) Quantification of cleavage defects in WT, MZttc28stl363/stl363, and MZdchs1b embryos at 3–3.5 hpf following DMSO control or 75 μM ZM447439 treatment. The right panel shows the representative severity of cleavage defects. N, number of embryos.
(C) Representative time-lapse still images of YCL microtubules in Tg[ef1α:dclk-GFP] transgenic WT embryos after DMSO or 400 μM ZM447439 treatment. Scale bar, 30 μm.
(D) Quantification of YCL microtubule density in DMSO control and ZM447439 (400 μM)-treated embryos. Error bars represent SEM. N, number of embryos.
(E) Models of Dchs1b interacting with Ttc28 and Aurora B to control microtubule dynamics and embryonic cleavages in zebrafish blastomeres of WT and MZdchs1b mutant embryos. Dchs1b, Ttc28, and Aurora B during cleavages. Dchs1b binds to Ttc28 at the membrane to limit its microtubule dynamics reducing activity in the cytoplasm. Potential direct or indirect effects of Ttc28 on Aurora B are also illustrated. Binding of Dchs1b to Aurora B promotes its activity stimulating microtubule dynamics. Aurora B has additional effects on early cleavages.
See also Video S2.
Reprinted from Developmental Cell, 45, Chen, J., Castelvecchi, G.D., Li-Villarreal, N., Raught, B., Krezel, A.M., McNeill, H., Solnica-Krezel, L., Atypical Cadherin Dachsous1b Interacts with Ttc28 and Aurora B to Control Microtubule Dynamics in Embryonic Cleavages, 376-391.e5, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell