ZFIN Image: Chen et al., 2018, Fig. 6

Image

Figure Caption

Fig. 6

ttc28 Genetically Interacts with dchs1b to Regulate Microtubule Dynamics

(A) Representative time-lapse still images of YCL microtubules in MZdchs1b single and MZdchs1b; MZttc28^{stl363/stl363} compound mutants in Tg[ef1α:dclk-GFP] transgenic background at 1.75–2.5 hpf. Scale bar, 30 μm.

(B) Quantification and comparison of YCL microtubule density in WT, MZdchs1b single, and MZdchs1b; MZttc28^{stl363/stl363} compound mutant at 1.75–2.5 hpf. Error bars represent SEM. N, number of embryos.

(C) Representative time-lapse still images of microtubule dynamics during cytokinesis in MZdchs1b single and MZdchs1b; MZttc28^{stl363/stl363} compound mutant embryos using Tg[ef1α:dclk-GFP]. Scale bar, 30 μm.

(D) Quantification of midzone microtubule assembly rates in WT, MZdchs1b single, and MZdchs1b; MZttc28^{stl363/stl363} compound mutant blastulae at 1.75–2.5 hpf. The Y axis shows the ratio of midzone microtubule width d at each time point divided by the initial width d’. ^∗∗∗∗p < 0.0001, paired Student's two-tailed t test. Error bars represent SEM. N, number of embryos.

(E) Comparison of mitotic events in WT, MZdchs1b single, and MZdchs1b; MZttc28^{stl363/stl363} compound mutant embryos at 3 hpf. N, number of embryos; n, number of cells.

See also Video S8.

Figure Data

Phenotype details

Acknowledgments

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Reprinted from Developmental Cell, 45, Chen, J., Castelvecchi, G.D., Li-Villarreal, N., Raught, B., Krezel, A.M., McNeill, H., Solnica-Krezel, L., Atypical Cadherin Dachsous1b Interacts with Ttc28 and Aurora B to Control Microtubule Dynamics in Embryonic Cleavages, 376-391.e5, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell