ZFIN Image: Kontarakis et al., 2018, Fig. S12

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Figure Caption

Fig. S12

Early events of venous and lymphatic development in mir-126 and spred1 TP morphants. (A) Emergence of prox1⁺ cells in mir-126 (16 ng) and spred1 TP (800 pg) morphants at 36 hpf in the TgBAC(prox1a:KalT4-UAS: uncTagRFP) line. No observable reduction in TgBAC(prox1a:KalT4-UAS: uncTagRFP)+ cells was found between treatments; confocal pictures of live imaging; zero to three cells were found in the ventral part of the PCV in each embryo analysed; Error bars are SEM. (B) Secondary sprouting analysis in a Tg(lyve1:DsRed2) mir-126a^+/-; mir-126b^-/- incross at 48 hpf, after immunostaining for DsRed. Similar to our observations at 5 dpf, mir-126 mutants display secondary sprouts from the PCV; two independent crosses. Error bars are SEM.

Acknowledgments

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Reprinted from Developmental Biology, 437(2), Kontarakis, Z., Rossi, A., Ramas, S., Dellinger, M.T., Stainier, D.Y.R., Mir-126 is a conserved modulator of lymphatic development, 120-130, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.