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Fig. S3
Epithelial markers are not expressed in the central osteoblasts. (A–C) Scales of M848:GFP fish were stained with antibodies against pan-cadherin (A), α-catenin (B), and β-catenin (C). None of these antibodies labeled the cell boundaries of the central osteoblasts. (A′–C′) Optical cross-sections at a thickness of 58.9 µm (A′), 55.4 µm (B′), and 65.8 µm (C′). (A″–C″) Higher magnifications for the central cells. Scale bars, 20 µm (A–C), 10 µm (A″–C″).
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Reprinted from Developmental Biology, 437(2), Iwasaki, M., Kuroda, J., Kawakami, K., Wada, H., Epidermal regulation of bone morphogenesis through the development and regeneration of osteoblasts in the zebrafish scale, 105-119, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.