Fig. 7

Fig. 7

Effects of cyclopamine and BMS-833923 on scale regeneration. (A,B) Cyclopamine treatment during scale regeneration over the 1–4 days after scale removal severely affects scale morphology (B) compared with control (A). Three consecutive scales were removed. (B′) Higher magnification image of the area shown in (B). The regenerated scales exhibit ill-defined edges, accompanied by a loss of posterior grooves (radii) and marginal cells; a few unaffected marginal cells are indicated by the arrowhead. GFP is strongly expressed in the regenerated scale due to an increase in the number of central cells (see Fig. 4K). (C–E) Quantification of scale abnormalities in cyclopamine- and BMS-833923-treated fish indicates a marked reduction in scale area (C), a marked reduction in marginal cells (D), and a slight reduction in central cells (E). (F,G) Cyclopamine did not induce apoptotic cell death, as revealed by acridine orange staining. Data are the mean± s.d. *P < 0.05 (t-test). Scale bars, 500 µm (A,B), 100 µm (B′,F,F′).