ZFIN Image: Iwasaki et al., 2018, Fig. 6

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Figure Caption

Fig. 6

Expression of shha in the epidermal cells overlying marginal osteoblasts. (A–D) M848:GFP juvenile fish were double stained with anti-GFP antibody (green) and anti-sense shha RNA probe (magenta). A prospective scale area is indicated by the dotted line in (A). The expression domain of shha becomes restricted to the posterior part of the scale when M848:GFP is expressed (B,C). Arrows in (B) indicate GFP-expressing cells. (D) Higher magnification image of the area shown in (C). (D′) Optical cross-section along the dashed line in (D) at a thickness of 21.1 µm. shha is expressed in arrays of cells adjacently overlying the marginal cells (arrowheads). Scale bars, 50 µm (A–C), 10 µm (D).

Acknowledgments

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Reprinted from Developmental Biology, 437(2), Iwasaki, M., Kuroda, J., Kawakami, K., Wada, H., Epidermal regulation of bone morphogenesis through the development and regeneration of osteoblasts in the zebrafish scale, 105-119, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.