ZFIN Image: Selland et al., 2018, Fig. S5

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Figure Caption

Fig. S5

Two-color analysis of rhombomere identity with fgf8, dusp6 and spry2.

The expression of representative markers is examined in combination with krox20. Fgf8, dusp6 and spry2 are shown in purple, while krox20 is shown in red. Krox20 expression labels r3 and r5. All embryos are dorsal views, anterior to the left, 4–5 somites. Fgf8 is expressed in r4, medial r2 and the MHB (A). The loss of hoxb1a reduces the space between r2 and r4 and the MHB becomes arrowhead shaped (B). In hoxb1b^−/− mutants (C) and hoxb1a^−/−hoxb1b^−/− mutants (D) there is ectopic expression of fgf8 in r3 and reduced expression in the lateral portions of r4. The expression of FGF responsive genes, dusp6 (E-H), and spry2 (I-L), are unaltered from wildtype (E,I) in hoxb1a^−/− mutants (F,J). In both hoxb1b^−/− mutants (G,K) and hoxb1a^−/−;hoxb1b^−/− mutants (H,L) there is an increase in expression levels. Spry2 shows ectopic expression in r3 (K,L). All embryos have been genotyped for hoxb1a^sa1191 and hoxb1b^ua1006. All images are dorsal views, anterior to the left, 4 somites.

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Acknowledgments

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Reprinted from Mechanisms of Development, 150, Selland, L.G., Koch, S., Laraque, M., Waskiewicz, A.J., Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development, 28-41, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.