Fig. S2

Fig. S2

Expression of hsp90aa1.2 mRNA in PGCs and in somatic cells. (A) The relative levels of hsp90aa1.2 and nanos3 in PGCs compared with the expression level in somatic cells at 7 and 36 hpf as determined using microarray-based analysis. (B) Number of reads obtained in high throughput sequencing of sorted PGCs and somatic cells at 4 and 7 hpf (at the onset and during PGC migration respectively) for hsp90aa1.2 and nanos3 . (C) Results of RT-PCR of hsp90aa1.2 and nanos3 using cDNA from sorted 7 hpf somatic cells and PGCs. (D) RT-PCR analysis demonstrating hsp90aa1.2 mRNA expression in embryonic, juvenile and adult zebrafish stages. (E) RNA containing the 3'untranslated region of hsp90aa1.2 and the open reading frame of the gene fused to that of mCherry injected into early embryos is preferentially translated in GFP-F labeled PGCs. Magnified images of PGCs are provided in the middle panels and the positions of the PGCs are marked with arrowheads in the low magnification panels. Asterisks label the region where the gonad develops.