Fig. S2
Maternal zygotic (MZ)udu-/- embryos specify all three germ layers
Whole mount in situ hybridizations (WISH) in WT (top row) and MZudu-/- embryos (bottom row) at the stages indicated. a-b) ntla marks nascent mesoderm. c-d) gsc and eve1 mark the embryonic shield and ventral mesoderm, respectively. e-f) sox17 marks endoderm and dorsal forerunner cells. g-j) shh (g-h) and ntla (i-j) mark axial mesoderm. Fractions indicate the number of embryos with the pictured phenotype over the number of embryos examined. Images depict a dorsal view with anterior/animal pole up in all images except c-d in which dorsal is to the right. Scale bar is 300μm.