Fig. 7
DHA rescues hematopoiesis in apoc2 mutants. a?c apoc2 mutant embryos were injected with free fatty acid docosahexaenoic acid (DHA), oleic acid (OA), or eicosapentaenoic acid (EPA) at 48?hpf. a o-Dianisine staining of 6.3 dpf larvae. b Representative bright field images and quantitative results of blood cell (yellow arrows) count in the caudal vein (outlined with white dashed lines) at 6.3 dpf. Mean?±?SEM; n?=?10 (WT), n?=?8 (apoc2 mut, apoc2 mut?+?OA, and apoc2 mut?+?EPA), and n?=?9 (apoc2 mut?+?DHA). ***P?<?0.001 (Student?s t test). c In situ hybridization with cmyb/runx1 and ?-globin probes. Scale bars, 100??m in a; 50??m in b; and 200??m in c