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Fig. S3
The splicing factor mutant sf3b1hi3394 have elevated levels of H3S10P.
(A) Embryos were analyzed at 31 hpf by whole-mount immunofluorescence to detect histone H3 phosphorylation at serine 10 (H3S10P). Arrowheads mark increased H3S10P staining in the head of the sf3b1hi3394aTg mutants. Magnified representative images of neural tissue highlighting differences in staining. (B) Neural tissue from the tails of sf3b1hi3394aTgmutants and siblings was quantified for intensity of H3S10P staining and normalized to the average number of H3S10P positive puncta per sibling control.
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Full text @ Dis. Model. Mech.