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Fig. S11
Clonal stab2 deletion. Whole-embryo and cellular views of DOPG liposome distribution in stab2ibl2/+; kdrl:GFP+ embryos. CRISPR/Cas9 introduced mutations in the wildtype allele generated clones of cells without stab2 function. In stab2 sgRNA and Cas9 mRNA injected, but not control embryos, endothelial cell clones are observed in which DOPG accumulation is abrogated, indicating a requirement for stab2 function within SECs.
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