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Fig. S10
Gene expression in stab2ibl2 homozygous mutant embryos. Whole-mount in situ hybridization of stab1 and mrc1 mRNA expression at 56hpf and stab2 mRNA expression at 32hpf and 56hpf in the trunk and tail of stab2ibl2 homozyogous mutant and sibling control embryos. A reduction in stab2 expression indicating nonsense-mediated decay of the stab2ibl2 mRNA is observed stab2ibl2 homozygous, but not sibling control embryos. Expression of stab1 and mrc1 is unchanged indicating normal SEC differentiation.
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