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Fig. 6
Analysis of Tbx2b and closure of the superior ocular sulcus.
(A) Whole-mount in situ hybridization for zebrafish tbx2b in control and BMP-depleted embryos. Top panels are eyes dissected from control and DMH1-treated embryos; bottom panels are from gdf6a+/+, and gdf6a-/- embryos. (B-C) Analysis of SOS closure in Tbx2b-depleted embryos. DIC images of eyes from live tbx2b+/+ (top panel) and tbx2bfby (bottom panel) embryos (B). Quantification of SOS closure in wild type and tbx2bfby mutant zebrafish eyes (C). Data are means ± SEM; one-way ANOVA with Tukey’s test: *P<0.05. Scale bars are 50 μm.
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