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Fig. 5
MiR-194 promoted the differentiation of the sensory patches in zebrafish inner ear. The percentage of Sox2 (upper portion) and acetylated-tubulin (lower portion) positive cells were measured by in situ hybridization at 48 hpf. The white circle area indicated zebrafish inner ear. Red staining indicated Sox2 expression (upper portion) or acetylated-tubulin expression (lower portion); blue indicated cell nucleus. MO – microinjection with morpholino oligonucleotide; NC – negative control; Mimics – microinjection with miR-194 mimics; hpf – hour post fertilization.
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