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Fig. S6
Quantification of muscle birefringence
(a-b) Birefringence of skeletal muscle at 48 hpf in wild type embryos exposed to vehicle control or BPA at 6 hpf. c) Wild type embryos exposed to vehicle control or 50 μM BPA had similar skeletal muscle birefringence at 48 hpf (P=0.27; N=5 biological replicates, N=1 technical replicate). Birefringence values represent the mean intensity of the total trunk normalized to the area of trunk analyzed. Scale bar = 500 μm. Data was analyzed by students t-test using Welch’s correction.
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