Fig. 5

Fig. 5

sox2andsox3do not directly specify neural or sensory fates. (A–F) Dorsolateral views (anterior to the left) showing otic expression of atoh1a at 16.5 hpf (A–C) and 18.5 hpf (D–F) in control (A, D), hs:sox2/hs:sox2 (B, E) and hs:sox3/hs:sox3 (C, F) embryos following heat-shock at 12.5 hpf. (G–L) Dorsolateral views showing otic expression of neurog1 at 16.5 hpf (G–I) and 18.5 hpf (J–L) in control (G, J), hs:sox2/hs:sox2 (H, K) and hs:sox3/hs:sox3 (I, L) embryos following heat-shock at 12.5 hpf. Otic vesicle borders are outlined in all images. (M, N) Quantification of the number of phospho-Histone H3 positive (PH3+) cells in the otic vesicle of control and hs:sox2/hs:sox2 (M) or hs:sox3/hs:sox3 (N) embryos at multiple time points following heat-shock at 12.5 hpf. Error bars represent standard deviation. No statistically significant differences between control and hs:sox2/hs:sox2 or hs:sox3/hs:sox3 embryos at any time point examined (student's t-test, n>15). In all panels, hs:sox2/hs:sox2 embryos were heat shocked at 39 °C for 60 min, whereas hs:sox3/hs:sox3 embryos were heat shocked at 38 °C for 30 min.