IMAGE

Fig. 5

ID
ZDB-IMAGE-180620-20
Source
Figures for Auer et al., 2018
Image
Figure Caption

Fig. 5

Targeted Ablation of Individual Oligodendrocytes without Causing Bystander Axon Damage

(A) Schematic drawing of single oligodendrocyte ablation using two-photon bleaching of mbp:KillerRed-labeled cells.

(B) Timeline of confocal images of individual mbp:KillerRed-expressing oligodendrocytes in Tg(mbp:EGFP-CAAX) full transgenic zebrafish at 7 days post-fertilization (dpf) before and after two-photon bleaching of the cells marked by the asterisk. Scale bars, 10 μm (left), 5 μm (right).

(C) Confocal images and axon reconstructions of huC:tagCFP-labeled axons in Tg(mbp:KillerRed) zebrafish before and after oligodendrocyte ablation at 4 dpf. Scale bar, 10 μm.

(D) Quantification of the beginning of cell body disintegration after two-photon bleaching of mbp:KillerRed-expressing cells at 4 dpf. Data are expressed and mean ± SD.

(E) Quantification of traced neurites in direct proximity to the ablation site before and after oligodendrocyte ablation as shown in (C).

See also Figure S3 and Movie S3.

Acknowledgments
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