|
Fig. 6-S1
Needle damage of the entpd5a cell domain leads to supernumerary vertebrae.
(a–c) Images of 5dpf Tg(entpd5a:kaede) embryos that were injured between entpd5-expressing segments. (d) Alizarin red staining of adult vertebrae from 5 dpf larvae injured between entpd5-expressing segments from a). Arrow points at site of injury. n = 5/5 when injury was done at 5dpf and 6/6 when injury was done at 7dpf e–g). Images of 5dpf Tg(entpd5a:kaede) embryos that were injured in the middle of the entpd5-expressing segments. (h) Alizarin red staining of adult vertebrae from 5 dpf larvae injured in the middle of entpd5-expressing segments from e) Arrow points at site of injury, and indicates extra vertebra. n = 1/1 for fish injured at day 5, and n = 3/3 for fish injured at day 7 pf. The scale bar in panel a,e is 100 µm and applies to b, c, f and g. The scale bars in d and h are 1000 µm.