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Fig. 7
Confirmation of differential gene expression using dual fluorescent in situ hybridization.
(A) Dual-FISH of nrp1a with an or111 sub-family probe cocktail. Two cells have been highlighted to show that nrp1a signal (red) is localized in cells with or111 subfamily labelling (green). (B) Summary of dual-FISH expression for four candidate guidance receptors. The percentage of OSNs expressing the indicated OR subfamily that also expressed the indicated candidate guidance transcript are noted. The total number of OR subfamily expressing cells counted are shown in parentheses. Pink shading indicates transcripts that showed higher overlap with the or111 sub-family probe-mix and green shading indicates transcripts that showed higher overlap with or133 sub-family probe mix. nrp1a shows greater overlap with or111 subfamily expression than with or133 subfamily expression. nrp1b, pcdh11, and robo2 show greater overlap with or133 subfamily expression than with or111 subfamily expression.