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Fig. 5-S3
Jupa knockdown results in reduced E-cadherin levels at lateral membranes of KV cells.
Immunostaining with E-cadherin (E-cad) antibodies showed E-cad enrichment at lateral membranes of KV cells marked by membrane-targeted GFP expression in Tg(sox17:GFP-CAAX) embryos. Embryos injected with jupa MO-1 showed a moderate reduction of E-cad protein enrichment at KV membranes. Arrows point to representative lateral membranes in individual channels. The graph represents normalized E-cad intensities from two experiments. N = number of embryos analyzed. Scale = 5 μm. *p<0.05 (Welch’s T-Test).
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