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Fig. S1
Antibody against human WWTR1 appears to be specific for zebrafish Wwtr1. (A) Maximum intensity projections of cardiac ventricles stained for Wwtr1. Punctae observed in wwtr1-/- samples are non-specific signal. Scale bars, 10 μm. Other groups have also shown that this antibody is specific for zebrafish Wwtr1 in other tissues (Miesfeld et al., 2015; Kimelman et al., 2017). (B) Quantification of Wwtr1 staining intensity in the nuclei of trabecular cardiomyocytes relative to the nuclei of adjacent compact cardiomyocytes at 60 and 72 hpf. ‘n’ number of trabecular cardiomyocyte nuclei were measured from 4 hearts at each time point. Error bars are one unit of standard deviation. ** - P < 0.01; *** - P < 0.001 by two-sample t-test.