ZFIN Image: Sánchez-Iranzo et al., 2018, Fig. S15

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Figure Caption

Fig. S15

tbx5a-derived cardiomyocytes within the cortical layer are lama5 positive.

lama5 RNAScope in situ detection followed by GFP and Myosin Heavy Chain (MHC) immunofluorescence on sagittal sections of tbx5a:GFP (a–h) or tbx5a:mCherryp-2a-CreER^T2;ubb:loxP-lacZ-loxP-GFP double transgenic animals, recombined before injury and fixed at 90 days postinjury (dpi) (i-p). Nuclei are counterstained with DAPI. a–d Uninjured heart. tbx5a:GFP (green) does not co-localize with lama5 (red). lama5 is expressed at higher and more homogenous levels in the cortical layer (n=4/4). e–h Uninjured heart. anti-MHC marks cardiomyocytes (green). In the cortical layer, regions of lama5/MHC co-localization were detected (white arrowheads) (n=4/4). i–p 90 dpi regenerated hearts. tbx5a:mCherryp-2a-CreER^T2;ubb:loxP-lacZ-loxPGFP were treated with two 12 hours pulses of 4-Hydroxytamoxifen (4-OHT) at 6 and 7 days before the injury. GFP⁺ cells marking the tbx5a lineage within the cortical layer were positive for lama5 (yellow arrowheads); double positive cells were observed in 11 out of 11 hearts. GFP⁺ cells within the trabecular layer were negative for that marker (green arrowheads; n=11/11). at, atrium; ba, bulbus arteriosus; v, ventricle. Scale bars, a, e, i 100 μm, c, f, j, n 25 μm

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