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Fig. 3-S1
Regulation of wu:fc23c0 by Yap1 and Wwtr1.
(A,B) wu:fc23c0 is strongly expressed throughout the notochord of sibling embryos (A) but not expressed in the posterior notochord of yap1;wwtr1 double mutant embryos. (C–E) Transgenic embryos obtained from an outcross of hemizygous HS:DN-yap or HS:CA-yap adults were heat shocked at the 4-somite stage and then grown to 18 somites. The genotype of the embryos was determined by PCR after the in situ hybridization to be either transgenic or non-transgenic control. Expression of DN-yap decreased posterior notochord wu:fc23c0 expression (D, n = 10) whereas CA-yap did not appear to alter wu:fc23c0 expression (E, n = 8) relative to control non-transgenic embryos (C).