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Fig. 5
Left-sided asymmetric expression of RUVBL1 at the mouse embryonic node: (A) whole-mount in situ hybridization (WISH) of Ruvbl1 probes for whole-mount head-fold stage wild-type C3H/HeH mouse embryos showing widespread, ubiquitous Ruvbl1 expression and left-sided asymmetric distribution at the embryonic node. Grey frame indicates magnified inset (lower left) with a second example at lower right, with Ruvbl1 expression indicated by arrowheads. Scale bar = 100 μm. (B) Light-sheet microscopy images of whole-mount immunofluorescence for RUVBL1 (red) and DAPI (blue) in the wild-type C57BL/6 mouse E7.25 and E7.5 (late head fold and 2–4 somite stage) embryonic node, and E9.0 (6–8 somite stage) vasculature and heart loop. Left (L) and right (R) sides are indicated. RUVBL1 is asymmetrically distributed at the murine embryonic node (upper two panels). RUVBL1 is present in a widespread distribution throughout the atrial chambers, primitive ventricle and developing vasculature (lower two panels). Scale bars = 100 or 200 μm, as indicated. Abbreviations: rAC and IAC, right and left atrial chambers; EN, embryonic node; L, left; N, notochord; PV, primitive ventricle; R, right; ST, septum transversum.