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Figure Caption
Fig. 2-S1
Yap1 localizes to the presumptive epidermis.
A combination of FISH and immunofluorescence was used to co-stain embryos for Yap1 protein and keratin 4 or keratin 8 mRNA, which are restricted to the presumptive epidermis. A brief (5 min) Proteinase K step was used for the FISH, which preserves the presumptive epidermal Yap1 stain but does not allow for strong notochord staining.
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