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Fig. 5
The effects of leucine treatment on autophagy in zebrafish testis. (A) The mRNA expression of autophagy related genes atg5, atg7, atg12 and p62 were evaluated by qRT-PCR. (B) Representative immunoblots showed P62 and Lc3 proteins after leucine and LY294002 administration. Actin was used as the control. Relative protein levels of P62 and Lc3-II were shown from 3 independent experiments. (C) Representative immunofluorescence pictures to detect Lc3 levels in control and leucine-treated zebrafish testes. Scale bar: 50 μm. Bars represent mean ± SEM of more than three separate experiments. *P < 0.05, **P < 0.01 and ***P < 0.001.