ZFIN Image: Berger et al., 2018, Fig. S5

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Figure Caption

Fig. S5

TEM micrographs reveal a reduced amount of myofibril in cct mutants. Related to Figures 1 and 2.

(A-E) Sarcomere organization was preserved within all analyzed cct mutants as revealed by EM micrographs of 3-dpf-old larvae. Boxed areas within micrographs of cct mutants are magnified. Arrows point to Z-disk-associated t-tubules and arrowheads notify aggregates. Representatives of a minimum of 3 analyzed larvae per genotype are shown (n > 3 per genotype). (A) The structure of sarcomeres was preserved in cct1^hi3564Tg, however the amount of myofibril was reduced in cct1^hi3564Tg homozygotes. (B) The reduction in the amount of myofibril and the preserved sarcomere organization was also apparent on TEM micrographs of cct2^hi1269Tg. (C) Also in cct5^hi2972Tg mutants, sarcomere organization was preserved, but the amount of myofibril was reduced. (D) cct8^mn30Gt mutants showed a similar myofibril reduction and unaffected sarcomere organization. (E) In cct4^-14 homozygotes the amount of myofibril was reduced and electron-dense aggregates close to Zdisks were documented (arrowheads).

Acknowledgments

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