ZFIN Image: Berger et al., 2018, Fig. S4

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Figure Caption

Fig. S4

In skeletal muscle, mutations within cct genes can lead to enlarged regions of endomysial connective tissue with reduced amount of myofibril. Related to Figures 1 and 2.

(A-G) Cross sections of 3-dpf-old larvae stained for H&E indicate enlarged regions of endomysial connective tissue in cct mutants compared to their siblings. Representatives of a minimum of 6 analyzed larvae per genotype are shown (n > 6 per genotype). (A) Homozygotes of gene trap mutant cct1^hi3564Tg and siblings. (B) Homozygotes of gene trap mutant cct2^hi1269Tg and siblings. (C) Homozygotes of the mutant cct5^tf212b and siblings. (D) Homozygotes of gene trap mutant cct5^hi2972Tg and siblings. (E) Homozygotes of gene trap mutant cct8^mn30Gt and siblings. (F) Homozygotes of null mutant cct3^sa1761 and siblings. (G) Homozygotes of null mutant cct4^-14 and siblings. (H and I) Representatives of 4 analyzed larvae per genotype are shown (n = 4 per genotype). (H) The reduced amount of myofibril in cct3^sa1761 mutants was confirmed in the transgenic background of Tg(acta1:mCherryCaaX) and Tg(acta1:liveact-GFP). (I) According to the reduced level of birefringence, a reduced amount of myofibril in cct4^-14 was visualized by Tg(acta1:mCherryCaaX) and Tg(acta1:liveact-GFP). (J) Regularly spaced actinin-positive Z-bodies of pre-myofibril were detected in siblings as well as in cct4^-14 homozygotes.

Acknowledgments

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