|
Fig. 2
MSI-1436 stimulates adult zebrafish heart regeneration. a Representative images of cardiomyocyte proliferation 3 days post-amputation (dpa) of the ventricular apex in vehicle-treated fish and MSI-1436-treated fish. Arrows show proliferating cardiomyocytes expressing Mef2 and PCNA. b Quantification of proliferating cardiomyocytes 3?dpa expressed as percentage of cells Mef2-positive cells and PCNA-positive cells relative to cells expressing Mef2 only. Values are means?±?S.E. (n?=?10?12). *P?<?0.01 compared to vehicle-treated fish. c Representative images of Tropomyosin expression in regenerating heart tissue 21?dpa. d Quantification of Tropomyosin expression and injury area 21?dpa. Values are means?±?S.E. (n?=?8?10). *P?<?0.01 compared to vehicle-treated fish. e Representative images of Tg(gata4:GFP) expression in regenerating hearts at 21?dpa. f Quantification of Tg(gata4:GFP) expression and the injury area at 21?dpa. Values are means?±?S.E. (n?=?4-6). *P?<?0.05 compared to vehicle-treated fish. Fish were given daily given intraperitoneal (IP) injections of either vehicle, 0.125?mg/kg MSI-1436 or 0.125?mg/kg squalamine (squa) following ventricular resection. Dashed lines show approximate resection plane