Fig. 7

Transcriptome Analysis of Dnd-Depleted PGCs Reveals a Transformation to a Somatic Transcription Profile

(A) A scheme depicting the flow of the transcriptome analysis of PGCs sorted at 13 hpf based on specific expression of EGFP (green label).

(B) Upregulated transcripts (up) in PGCs knocked down for Dnd are enriched for gene ontology (GO) terms involved in somatic tissue developmental processes (upper graph). Representative terms with a minimum log₁₀ p value of −15 are presented in this graph. Downregulated transcripts (down) showing enrichment for germline-related processes (lower graph) relative to control PGCs. Depicted are GO terms with at least 5-fold enrichment relative to control cells.

(C) A table presenting selected genes from the list of up- and downregulated transcripts in Dnd-deficient PGCs relative to control cells. The list of downregulated genes includes examples of seven genes involved in germline development; the upregulated genes listed are examples of genes important for the development of different germ layers.

KD, knockdown; hpf, hours post fertilization; FACS, fluorescence-activated cell sorting; mRNA seq, mRNA sequencing; FPKM, fragments per kilobase per million reads; Ctr, control; dnd, Dead end knockdown; FC, fold change. See also Table S1 (Chong et al., 2001; Doitsidou et al., 2002; Guo et al., 1999; Houwing et al., 2008; Jiang et al., 2009; Köprunner et al., 2001; Mishima et al., 2006; Strasser et al., 2008; Tarbashevich et al., 2015; Weinberg et al., 1996; Yoo et al., 2003; Yoon et al., 1997; Zhang et al., 2004).