ZFIN Image: Gross-Thebing et al., 2017, Fig. 5

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Figure Caption

Fig. 5

Reprogramming of PGCs to Endodermal Fate

(A) Expression of EGFP under the control of the endodermal promoter sox17 in Dnd-deficient PGCs misexpressing the RNA encoding for the constitutively active form of TaramA (taramA^∗). Arrowhead points at a PGC (identified by red membrane label, mCherry-F′) expressing EGFP. Directional cell migration was inhibited by knockdown of Cxcl12a. The farnesylated mCherry also labels the position of the Golgi apparatus within the PGCs. Confocal plane images (5 μm thick) were acquired at 10 hpf. Scale bars, 20 μm.

(B) The number of PGCs expressing EGFP under the control of the sox17 promoter in Dnd-depleted PGCs expressing a constitutively active form of TaramA (taramA^∗) at 10 hpf.

N, number of embryos; n, number of cells; F′, farnesylated; KD, knockdown; hpf, hours post fertilization.

Acknowledgments

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Reprinted from Developmental Cell, 43, Gross-Thebing, T., Yigit, S., Pfeiffer, J., Reichman-Fried, M., Bandemer, J., Ruckert, C., Rathmer, C., Goudarzi, M., Stehling, M., Tarbashevich, K., Seggewiss, J., Raz, E., The Vertebrate Protein Dead End Maintains Primordial Germ Cell Fate by Inhibiting Somatic Differentiation, 704-715.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell