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Fig. S3
Related to Figure 3. Apoptosis of Dnd-depleted PGCs (A) Germ cell death in time-lapse movies captured by light microscopy between 12 and 16 hpf in control and in embryos knocked down for Dnd. In both cases, directional cell migration was inhibited by knockout of the cxcr4b gene. The asterisk marks PGCs undergoing apoptosis (red boxes). In Dnd-deficient embryos the PGCs are immotile and some are undergoing morphological transdifferentiation (green boxes, arrows point at cells). Embryos are oriented with anterior up. See also movie S2. (B) The number of PGC apoptosis events in 12 to 16 hpf cxcr4b-/- embryos injected either with low (20 μM) or high (60 μM) amounts of dnd Morpholino. p-values were determined using the Mann-Whitney-U test with α=0.05 and corrected for multiple comparison. Data are presented as median ± IQR. (C) Mosaic labeling of somatic cells (green) in the neural plate (red box) in 14 hpf wildtype zebrafish embryos. 20 μm thick confocal plane image of somatic cells labeled with EGFP-ras. Scheme shows the embryo from dorsal view with the location of the imaged cells marked. Scale bar, 50 μm. Number of embryos (N), Farnesylated (F’), Knockdown (KD), hours post fertilization (hpf), Neural Plate (NP), Somites (S).
Reprinted from Developmental Cell, 43, Gross-Thebing, T., Yigit, S., Pfeiffer, J., Reichman-Fried, M., Bandemer, J., Ruckert, C., Rathmer, C., Goudarzi, M., Stehling, M., Tarbashevich, K., Seggewiss, J., Raz, E., The Vertebrate Protein Dead End Maintains Primordial Germ Cell Fate by Inhibiting Somatic Differentiation, 704-715.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell