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Fig. 4
grhl2b/grhl3 double morphants present with phenotypes highly consistent with disrupted convergence-extension mediated migration, as well as MHB folding defects. (a–i) MO:grhl2b + MO:grhl3 injected double morphants (a) display a reduced axial length and decreased length:width ratio (LWR) relative to embryos injected with MO:control (b). These differences were quantitated (c), showing significantly decreased LWR in MO:grhl2b + MO:grhl3 injected double morphants (p < 0.0001 by Student’s T-test). (d–g) Dorsal views of MO:grhl2b + MO:grhl3 double morphants (d,f) relative to MO:control embryos (e,g) at 24 hpf highlighting the thicker trunk, broadened notochord (n) and splayed somites (s) characteristic of embryos with disrupted convergence-extension; as well as disruption of MHB morphogenesis (compare f with g). (h–i) MO:grhl2b + MO:grhl3 double morphants (f) relative to MO:control embryos at 96 hpf, highlighting severely reduced axial extension.