ZFIN Image: Hui et al., 2017, Fig. S4

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Figure Caption

Fig. S4

Regeneration factor administration and receptor gene expression in regenerating parenchymal cells, Related to Figure 6. (A) NTF3 treatment regimen. NTF3 or PBS was injected intraperitoneally (i.p.) from 6 dpi. (B–E) Proliferation of neural progenitor cells (B; Sox2⁺PCNA⁺), immature neurons (C; HuC/D⁺EdU⁺), cardiomyocytes (D; Mef2⁺PCNA⁺) and Müller glia (E; gfap:GFP⁺PCNA⁺). Insets show single-channel confocal slices (B, C, E) or epifluorescent images (D) of the demarcated regions. Dotted line, wound border plane. (F) Quantified proliferating immature neurons (HuC/D⁺EdU⁺) in C (mean ± SEM, n = 5). (G–I) Semi-qRT-PCR analysis of purified gfap:GFP⁺ cells from spinal cords (G; ependymo-radial glial cells), cmlc2:GFP⁺ cells from hearts (H; cardiomyocytes), and gfap:GFP⁺ cells from retinas (I; Müller glia). Confocal projections of z-stacks are shown, except for the epifluorescent images shown in D. *P < 0.05, **P < 0.01, Mann–Whitney U test. NS, not significant. Scale bars, 50 μm.

Acknowledgments

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Reprinted from Developmental Cell, 43, Hui, S.P., Sheng, D.Z., Sugimoto, K., Gonzalez-Rajal, A., Nakagawa, S., Hesselson, D., Kikuchi, K., Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs, 659-672.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell