ZFIN Image: Hui et al., 2017, Fig. 2

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Figure Caption

Fig. 2

Distribution and Characterization of Zebrafish foxp3a⁺ Cells

(A and B) foxp3a:RFP⁺ cells in lymphoid (A) and adult peripheral tissues (B).

(C and D) FACS analysis of total kidney cells from adult foxp3a:RFP (C) and lck:GFP;foxp3a:RFP fish (D). The percentage of cells in each gate are indicated in the plots (C; mean ± SD, n = 5). The lymphoid gate from (C) is shown in (D).

(E) Semi-qRT-PCR analysis of purified lck:GFP⁺foxp3a:RFP⁻ (upper-left gate in D) and lck:GFP⁺foxp3a:RFP⁺ cells (upper-right gate in D). Thymus (Thy) and kidney (Kid) cell suspension was used as control for T cell genes and non-T cell genes.

bd, bile duct; ep, epithelium; oe, olfactory epithelium; oc, oocyte; v, vein; FSC, forward scatter; SSC, side scatter. Scale bars, 50 μm.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Cell, 43, Hui, S.P., Sheng, D.Z., Sugimoto, K., Gonzalez-Rajal, A., Nakagawa, S., Hesselson, D., Kikuchi, K., Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs, 659-672.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell