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Fig. S6
The h2afv morphant phenotype is unaffected by co-injection with standard control morpholino.
(A) Fish embryos were injected with standard control MO 2, h2afv MO or standard control MO 2 + h2afv MO and were scored for phenotype at 24 hpf by bright field microscopy. Both the uninjected and standard control MO 2 were identical. Injection of h2afv MO along with the standard control MO 2 elicited the same phenotype as seen with h2afv MO alone, confirming that the phenotype is specifically due to the loss of H2afv protein. Scale bar: 1000 μm. (B) Analysis of the number of the h2afv morphants with and without addition of standard control MO 2 at 24 hpf. The control MO injection (0.67 ng) alone does not elicit any overt phenotype and its addition in h2afv morphants does not cause a change the phenotype caused by the h2afv morpholino.