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Fig. 1 Ethanol-induced cardiac chamber defects occurring during embryogenesis persisted in older zebrafish larvae. (A–C) 3D renderings of confocal sections of Tg(kdrl:EGFP) show closely attached atrium and ventricle residing side by side in the control larva (A); ventricles are on the top of the atria in ethanol-exposed larvae (B,C); Dotted yellow line represents the line through AV valves connecting atrium and ventricle. The angle between yellow line and antero-posterior axis is shown by white dots; (D–F) Masson’s trichrome stained histology sections at the atrioventricular canal region showing both atrium and ventricle revealed defective anatomy of the ethanol-exposed larvae (E,F) compared to control (D); (G–I) Masson’s trichrome stained histology sections of the ventricle showed healthy looking cardiac muscle (red colored trabeculae) in control larva (G); and myocardial damage (blue colored trabeculae, yellow arrows) in ethanol-exposed larvae (H,I).