IMAGE

Fig. 2

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ZDB-IMAGE-180417-20
Antibodies
Source
Figures for Rogers et al., 2017
Image
Figure Caption

Fig. 2

Lefty loss causes expanded Nodal signaling gradients.

α-pSmad2 staining (red) at sphere stage (A, animal views) and 50% epiboly (B, lateral views) in representative wild type and lft1-/-;lft2-/- mutant embryos (nuclei labeled with Sytox nuclear stain (green)). Note stronger pSmad2 staining in lft1-/-;lft2-/- mutant embryos compared to wild type. (C) Quantification of pSmad2 gradients in wild type (blue) and lft1-/-;lft2-/- (red) mutant embryos at dome stage (dashed) and 50% epiboly (solid) shows an increase in the amplitude and range of the signaling gradient in lft1-/-;lft2-/- mutants. See Materials and methods and Figure 2—figure supplement 1 for quantification details.

Figure Data
Acknowledgments
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