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Fig. 2 Cell death is increased in Nanog mutants. (A-C) Groups of Acridine Orange (AO)-stained dead cells in 12 hpf MZnanog (A), Mnanog (B) and wild-type (WT, C) embryos are visible as green spots. Yellow arrowheads and white arrows point to anterior and posterior ends of dorsal embryo axes, respectively. Scale bar: 100 µm. (D) The increase in cell death in MZnanog over Mnanog is statistically significant (P<10−6, Student's t-test); n, number of scored embryos. Error bars represent s.e.m.
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