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Fig. S10
Endothelial lineage cells are not involved in cardiomyocyte FATC reporter expression. Tg(ubb:FATC) ;Tg(kdrl:cre) animals in which cre was constitutively expressed in endothelial and hematopoietic progenitor cells showed no LIFEACT-GFP+ (green) cells in the myocardium. mCerulean expression (blue) confirmed the presence of the ubb:FATC transgene. LIFEACT-GFP induction was assessed in 3 and 5 dpf animals. Images are average intensity projections of 12 μm thick confocal stacks shown in ventral views, anterior up. 12 animals were examined at each developmental stage.
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