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Fig. s1

WISH expression patterns of the genes differentially expressed in “heartless”. The patterns are shown for Tg(cmlc2:mcherry-NTR) treated by DMSO (NTR+DMSO) and “heartless” larvae at 4.5 dpf for each gene. A. Liver genes. B. Liver/kidney genes. The renal expression of dpys in 4.5 dpf larva is shown by co-staining with a marker for proximal convoluted tubule, slc20a1a. C. Genes expressed in the liver/somite/others (pfkfb3) and the liver/others (angpt2a and fosl1a). D. Somite gene. qRT-PCR result (top graph) shows the higher expression of tgm2a at 3.5 dpf. E. Pancreas gene. F. Genes expressed in undefined tissues/organs (Others). Scale bars, 500 μm.

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